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8405

Wastewater Microscopy and Staining Techniques

3.0

Wastewater

Pennsylvania Rural Water Association

Joel Jordan

jjordan@prwa.com

8143539302 Ext. 201

Partner Website

Microbiology and staining course using hands-on exercises to promote the use of the microscope for process control and troubleshooting of the activated sludge process. Major components of the mixed liquor examination that are reviewed include: 1) bulk solution, 2) floc particles, 3) filamentous organisms, 4) protozoa and 5) metazoa. Filamentous organism topics that are reviewed include the relative abundance of filamentous organisms and identification of filamentous organisms. A detailed review of the use of protozoa as “bio-indicators” of the “health” of the activated sludge and the quality of the mixed liquor effluent is provided. Exercises performed include the preparation of wet mounts, stained wet mounts, smears and stained smears. Calibration of objective lenses for microscopic measurements is also performed.

Intermediate thru Advanced

Microscope for process control Major Organisms in Activated Sludge Activated sludge food chain Sample collection and storage Slide prep Microscope types and techniques Stains Components of a mixed liquor examination Protozoa as bio-indicators Hands on exercises Exams and Discussion

Wastewater treatment operators and lab techs

Classroom